|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 323-376 of human HIF-3 alpha|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 72 kDa.
Purity is >95% by SDS-PAGE.
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (also designated Arnt 1), EPAS-1 (also designated HIF-2 alpha) and HIF-3 alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP7; BHLHE17; Class E basic helix-loop-helix protein 17; HIF-3 alpha; HIF-3-alpha; HIF3 alpha 1; HIF3-alpha; HIF3-alpha-1; HIF3A; hypoxia inducible factor 3 alpha; hypoxia inducible factor 3, alpha subunit; hypoxia inducible factor 3a; hypoxia inducible factor three alpha; hypoxia-inducible factor 3 alpha; Hypoxia-inducible factor 3-alpha; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; MOP7; Neonatal and embryonic PAS protein; PAS domain-containing protein 7; PASD7
Gene Aliases: BHLHE17; HIF-3A; HIF3-alpha-1; HIF3A; IPAS; MOP7; NEPAS; PASD7
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