|Flow Cytometry (Flow)||1:10-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_0713341 (GeneID 54583)|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody does not react with mouse samples.
Suggested positive control: antigen standard for EGLN1 (transient overexpression lysate), human glioblastoma cells.
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C1ORF12; Chromosome 1 Open Reading Frame 12; EGL 9 homolog of C. elegans 1; Egl nine homolog 1; egl nine-like protein 1; egl-9 family hypoxia-inducible factor 1; EGLN 1 EGLN1; HIF Prolyl Hydroxylase 2; HIF-P4H 2; HIF-PH2; HIF-prolyl hydroxylase 2; HIFPH2; Hypoxia-inducible factor prolyl hydroxylase 2; P4H2; PHD2; Prolyl Hy; Prolyl hydroxylase domain-containing protein 2; SM-20; zinc finger MYND domain-containing protein 6
Gene Aliases: C1orf12; ECYT3; EGLN1; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD-2; PHD2; PNAS-118; PNAS-137; SM20; ZMYND6
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