|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human HLA-DRB3|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 30 kDa.
Purity is >95% by SDS-PAGE.
Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in mhc ii-a/b dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain ( beta-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DR beta 3 chain; DR7; DRB3; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR beta 3 chain; HLA class II histocompatibility antigen DRB1 7 beta chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA DR3B; Human leucocyte antigen DRB3; Major histocompatibility complex class II DR beta 3; MGC117330; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRB3; MHC class II HLA DR beta 3 chain; MHC class II HLA-DR beta 3 chain
Gene Aliases: DRB1; DRw10; HLA-DR1B; HLA-DR3B; HLA-DRB; HLA-DRB3; SS1
UniProt ID: (Human) P79483
Entrez Gene ID: (Human) 3125
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