|Flow Cytometry (Flow)||1:100|
|Immunocytochemistry (ICC)||0.05 µg/ml|
|Immunofluorescence (IF)||0.05 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||0.5-1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Bovine, Dog, Human, Mouse, Sheep, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between to amino acids 100-200 of the human HMGB1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is expected to cross react with porcine, equine and rabbit samples based on 100% sequence homology.
Suggested positive control: Hela whole cell extract, antigen standard for HMGB1 (transient overexpression lysate).
HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. These proteins (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins.
HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. They may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Amphoterin; Amphoterin antibody; Heparin-binding protein p30; high mobility group 1; High mobility group 1 antibody; High Mobility Group Box 1 antibody; High mobility group protein 1; High mobility group protein 1 antibody; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG3 antibody; Hmgb1; Sulfoglucuronyl carbohydrate binding protein
Gene Aliases: Ac2-008; amphoterin; BOS_12315; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1
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