|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||0.5-1 µg/ml|
|Tested Species reactivity||Bovine, Dog, Human, Mouse, Sheep, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200).|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. These proteins (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. They may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Amphoterin; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG1; HMG3; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein
Gene Aliases: Ac2-008; amphoterin; BOS_12315; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.