|Flow Cytometry (Flow)||1:500|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 1-100 of the human HP 1 alpha protein.|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody is expected to cross-react with mouse, Arabidopsis suecica, Rye, and Triticum aestivum based on sequence homology.
Suggested positive control: antigen standard for CBX5 (transient overexpression lysate), Hela whole cell extract.
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure.
High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen p25; Antigen p25 antibody; Cbx5 antibody; Chromobox homolog 5 (HP1 alpha homolog Drosophila) antibody; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; Chromobox protein homolog 5 ant; epididymis luminal protein 25; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha
Gene Aliases: CBX5; HEL25; HP1; HP1A
UniProt ID: (Human) P45973
Entrez Gene ID: (Human) 23468
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