|Western Blot (WB)||0.01-0.1 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (DRHKETLKSKTQ) corresponding to the C-terminus amino acids of HSD3B1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 3 beta- and steroid hydroxy-delta-5-steroid dehydrogenase; 3 beta-and steroid delta-isomerase 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; delta-5-3-ketosteroid isomerase; HGNC:5217; HSD3B; HSD3B1; HSDB3; Hydroxy-delta-5-steroid dehydrogenase; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; progesterone reductase; short chain dehydrogenase/reductase family 11E, member 1; steroid Delta-isomerase; Trophoblast antigen FDO161G
Gene Aliases: 3BETAHSD; 3BH; HSD3B; HSD3B1; HSDB3; HSDB3A; I; SDR11E1
UniProt ID: (Human) P14060
Entrez Gene ID: (Human) 3283
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