|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Guinea pig, Hamster, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant mouse Heat Shock Protein 25.|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-25494 detects Heat Shock Protein 25 from mouse, rat, porcine, guinea pig, and hamster samples.
PA1-25494 has been successfully used in immunoprecipitation, and Western blot procedures.
The PA1-25494 immunogen is recombinant mouse Heat Shock Protein 25.
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock proteins. One class of stress proteins, termed the "small heat shock proteins" is comprised of a diverse group of proteins from ~15 to >30kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated strwess protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, turmor promoter (e.g. phorbol esters) and calcium ionophores. The expression of a 24kDa protein was shown to correlate with steroid hormone receptor in cell lines, organs, and tumors. The 24kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that levels of the Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kDa protein 1; heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; heat shock 70kDa protein 1-like; heat shock protein 1-like; Heat shock protein beta-1; heat shock protein, 25 kDa; HSP 25; HSP 27; HSP25; HSP27; HSPB1; p25; truncated hsp25
Gene Aliases: 27kDa; Hsp25; HSP27; HSPB1
Molecular Function: chaperone
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.