|Western Blot (WB)||0.2-1 µg/ml|
|Tested Species reactivity||Bovine, Dog, Chicken, Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 350-400 of human Hsp60.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: HeLa, Jurkat cell lysate, Hela whole cell lysate, antigen standard for HSPD1 (transient overexpression lysate), Jurkat whole cell lysate.
HSP60, a 60KDa member of Chaperonin family, is a mitochondrial protein that participates in protein folding, transport and secretion. HSP60 is a multi-functional protein whose roles include accelerating the maturation of pro-caspase 3 during apoptosis, LPS-binding, acting as a ligand for innate immune system and also binding to the surface of innate immune cells and stimulating proinflammatory reactivity. It induces the expression of IL-12 and IL-15 and also induces release of TNF-alpha, NO and IL-6. Reports suggest interaction of Hsp60 with integrin molecules during metastasis. Mutations in Hsp60 gene cause autosomal recessive spastic paraplegia 13. Hsp60 has been found to be involved in development of auto-immune disorders such as Rheumatoid arthritis, insulin-dependent diabetes mellitus, systemic sclerosis and atherosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP-60; hsp60; HSP65; HuCHA60; mitochondrial; Mitochondrial matrix protein P1; Mytonin Chaperone 60; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13
Gene Aliases: BOS_1963; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; RCJMB04_7g5; SPG13
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