|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Bacteria|
|Published species reactivity||Bacteria , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Chlamydia trachomatis HSP60 protein.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-023 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects all three species of Chlamydia HSP60 with minimal cross-reactivity with Borrelia burgdorferi HSP60. MA3-023 does not react with E. coli GroEL.
MA3-023 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~60 kDa band representing HSP60 from C. trachomatis lysate.
The MA3-023 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of Chlamydia HSP60 amino acids 517-522.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 60 kDa heat shock protein; chaperonin 60; GROEL; heat shock protein 65; HLD4; HSP65; mitochondrial; mitochondrial matrix protein P1; Mytonin Chaperone 60; short heat shock protein 60 Hsp60s1; SPG13
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