|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
|Western Blot (WB)||See 56 publications below|
|Immunocytochemistry (ICC)||See 18 publications below|
|Miscellaneous PubMed (MISC)||See 7 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Non-human primate|
|Published species reactivity||Dog , Rat , Non-human primate , Human , Mouse , Guinea pig|
|Host / Isotype||Mouse / IgG3|
|Immunogen||Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues.
MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate. Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria.
mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock protein, 70 kDa 3; heat shock-induced protein; Hsp68; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70.1/HSP70.2; HSP70.3; HSP70I; HSP72; HSPA1; inducible heat shock protein 70
Gene Aliases: HEL-S-103; hsp68; HSP70-1; HSP70-1A; Hsp70-3; HSP70.1; Hsp70.3; Hsp70A1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSX70
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