|Flow Cytometry (Flow)||5 ug/ml|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Western Blot (WB)||0.5-1 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 27–169 of human HGF|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Hepatocyte growth factor (HGF) is a member of the plasminogen subfamily of S1 peptidases. The inactive polypeptide formed upon translation undergoes cleavage to produce a 69 kDa alpha-chain and 34 kDa beta-chain. A disulphide bond between the two chains makes active HGF. HGF is secreted by mesenchymal cells and stimulates mitogenesis, cell motility, and matrix invasion. It is involved in cellular growth and morphogenesis in a wide variety of tissue types. In humans, HGF is present on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DFNB39; F-TCF; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoietin-A; HGFB; HPTA; lung fibroblast-derived mitogen; Scatter factor; SF
Gene Aliases: DFNB39; F-TCF; HGF; HGFB; HPTA; SF
UniProt ID: (Human) P14210
Entrez Gene ID: (Human) 3082
Molecular Function: annexin calcium-binding protein calmodulin enzyme modulator hydrolase intracellular calcium-sensing protein peptide hormone protease protease inhibitor receptor serine protease signaling molecule
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