|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 45 of Human Histone H2A.x|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28785 targets Histone H2A.x in WB applications and shows reactivity with Human samples.
The PA5-28785 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 45 of Human Histone H2A.x.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Protein Aliases: H2A histone family, member X; H2A/X; H2AX; H2AX histone; Histone H2A.X; Histone H2AX
Gene Aliases: H2A.X; H2A/X; H2AFX; H2AX
UniProt ID: (Human) P16104
Entrez Gene ID: (Human) 3014