|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Dog, Chicken, Fruit fly, Human, Mouse, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 111-125 of human histone H2B [GenBank no. CAI19747.1]|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila.
Suggested positive control: Jurkat, PBMC.
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Humsn histone H2B is a 125 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CG17949-PA; H2B; H2B histone family, member F; H2B I; H2B II; H2B III; H2B IV; H2B VI; H2B VII; h2B-143; H2B/f; H2BFD; His2B:CG17949-PA; HIST1H3I; histone 1, H2bb; Histone H2B; Histone H2B 1/2/3/4/6; Histone H2B 3; histone H2B 7; histone H2B type 1; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; MGC125414; MGC125415; MGC125416; MGC9388; RP1-193B12.10
Gene Aliases: CG17949; CG33868; CG33870; CG33872; CG33874; CG33876; CG33878; CG33880; CG33882; CG33884; CG33886; CG33888; CG33890; CG33892; CG33894; CG33896; CG33898; CG33900; CG33902; CG33904; CG33906; CG33908; CG33910; Dmel\CG17949; Dmel_CG17949; fj05g08; H2b-143; H2B-I; H2B-II; H2B-III; H2B-IV; H2B-VI; H2B-VII; H2B.1; H2B/f; H2BFF; His2B; His2B:CG17949; His2B:CG33868; His2B:CG33870; His2B:CG33872; His2B:CG33874; His2B:CG33876; His2B:CG33878; His2B:CG33880; His2B:CG33882; His2B:CG33884; His2B:CG33886; His2B:CG33888; His2B:CG33890; His2B:CG33892; His2B:CG33894; His2B:CG33896; His2B:CG33898; His2B:CG33900; His2B:CG33902; His2B:CG33904; His2B:CG33906; His2B:CG33908; His2B:CG33910; HIST1H2BB; hist2h2l; wu:fj05g08; zgc:73093
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.