|ChIP assay (ChIP)||1.5 µg/10^6 cells|
|Flow Cytometry (Flow)||1:20|
|Western Blot (WB)||1:1,000 - 1:4,000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Dog, Chicken, Fruit fly, Horse, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the carboxy-terminal sequence of human histone H3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other histones.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CG5825-PA; CG5825-PC; CG5825-PD; H3 histone family, member A; H3a; H3L-like histone; His3.3A-PA; His3.3A-PC; His3.3A-PD; HIST1H3A; Histone 1; histone 1, H2ai; histone 1, H3a; histone cluster 1, H2ai; histone cluster 1, H2ai-like; Histone H3.1; histone H3.3; histone H3.3 variant; histone H3.3A; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; histone variant 3.3; histone variant H3.3
Gene Aliases: CG5825; dH3.3A; Dmel\CG5825; Dmel_CG5825; H3 histone; H3.3; H3.3A; H3/A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; His-3.3A; His3.3; Hist1h2ai; Hist1h2ail; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; PH3
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