|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Bovine, Human, Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length protein of Saccharomyces cerevisiae Histone H4 expressed in E. coli|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA5-19475 targets Histone H4 in Western blot and flow cytometry applications and shows reactivity with Bovine, Human, and Yeast samples.
The PA5-19475 immunogen is recombinant full length protein of Saccharomyces cerevisiae Histone H4 expressed in E. coli.
PA5-19475 detects Histone H4 which has a predicted molecular weight of approximately 14 kDa.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: H4 histone family, member A; H4.1; H4/A; H4FA; Hhf1p; HIST1H4A; histone 1, H4a; Histone H4; Histone H4A
Gene Aliases: H4FA; HHF1; HHF2; N2752; YBR009C; YBR0122; YNL030W
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