|Western Blot (WB)||2.5-5 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide from near the center of human Hrk.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
Despite its predicted molecular weight, Hrk often migrates at 12-15kDa in SDS-PAGE. A suggested positive control is mouse pancreas tissue lysate.
PA5-20235 can be used with blocking peptide PEP-0369.
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activator of apoptosis harakiri; activator of apoptosis Hrk; BCL2-interacting protein; BH3 interacting (with BCL2 family) domain, apoptosis agonist; BH3-interacting domain-containing protein 3; death protein 5; DP5, HARAKIRI; harakiri, BCL2 interacting protein (contains only BH3 domain); Neuronal death protein DP5
Gene Aliases: AI838259; BID3; DP5; HARAKIRI; HRK
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