|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 50-100 of human Hel-N1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 40 kDa.
Purity is >95% by SDS-PAGE.
The Elav-like genes encode for a family of RNA-binding proteins. Elav, a Drosophila protein and the first described member, is expressed immediately after neuroblastic differentiation into neurons and is necessary for neuronal differentiation and maintenance. Several mammalian Elav-like proteins, designated HuC, HuD and Hel-N1, are also expressed in postmitotic neurons. An additional mammalian homolog, HuR, which is also designated HuA, is ubiquitously expressed and is also overexpressed in a wide variety of tumors. Characteristically, these homologs all contain three RNA recognition motifs (RRM), and they specifically bind to AU-rich elements (ARE) in the 3'-untranslated region of mRNAs transcripts. ARE sites target mRNA for rapid degradation and thereby regulate the expression levels of genes involved in cell growth and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B); ELAV like 4; ELAV like neuronal protein 1; ELAV like protein 2; ELAV like protein 4; ELAV-like neuronal protein 1; ELAV-like neuronal protein 1 isoform Hel-N2; ELAV-like protein 2; ELAVL 2; ELAVL 4; ELAVL2; embryonic lethal, abnormal vision-like 2; HEL-N1; HELN1; Hu antigen B; Hu antigen D; HU-antigen B; HUB; HUD; Nervous system-specific RNA-binding protein Hel-N1; Nervous system-specific RNA-binding protein Mel-N1; RNA binding protein HuB
Gene Aliases: Elav2; ELAVL2; HEL-N1; HELN1; HUB; mel-N1
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