|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2.5 ug/ml|
|Western Blot (WB)||2-4 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 24 amino acid peptide near the center of human IEX1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is human brain tissue lysate.
PA5-20391 can be used with blocking peptide PEP-0508.
IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-a. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappa-B/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: anti-death protein; cAMP inducible gene 3; DADB-118P11.5, DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1; Differentiation-dependent gene 2 protein; expressed in pancreatic carcinoma; gly96, mouse, homolog of; Immediate early protein GLY96; Immediate early response 3 protein; immediately early gene X-1; PACAP-responsive gene 1 protein; Protein DIF-2; Protein PRG1; Radiation-inducible immediate-early gene IEX-1
Gene Aliases: AI663993; cI-3; DIF-2; DIF2; GLY96; IER3; IEX-1; IEX-1L; IEX1; PRG1
UniProt ID: (Human) P46695
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