|Flow Cytometry (Flow)||1/10|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/10 - 1/40|
|Western Blot (WB)||Assay Dependent|
|ChIP assay (ChIP)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting, and is reported to partically block binding of IGF-I and IGF-II to CD221.
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is pancreas or placenta. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.
CD221, a 155kDa receptor tyrosine kinase also known as Insulin-like growth factor I receptor (IGF-I Receptor), is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD221; IGF-I receptor; IGFIR; IGFR; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; soluble IGF1R variant 1; soluble IGF1R variant 2
Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
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