|ELISA (ELISA)||0.5-2.0 ug/ml|
|Functional Assay (FN)||0.67-1.0 µg/ml|
|Western Blot (WB)||0.1-0.2ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IGF-I.|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||4° C|
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended (unless the product will be used for functional studies). Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
This antibody may be used in indirect ELISA or as a capture reagent in sandwich ELISA with PA1-86912 as a detection reagent.
IGF-1 (Insulin-like Growth Factor I) is a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumour cell growth. IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IGF-I; IGF1A; IGFI; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin-C
Gene Aliases: IBP1; IGF-I; IGF1; IGFI; MGF
UniProt ID: (Human) P05019
Entrez Gene ID: (Human) 3479
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