|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues I(148) H Q D D F G E S K F I T V(161) of huamn IKAP.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
PA1-4163 detects IKAP in human samples.
PA1-4163 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~158 kDa protein representing IKAP in Interleukin-1-stimulated 293 cells.
The PA1-4163 immunogen is a synthetic peptide corresponding to residues I(148) H Q D D F G E S K F I T V(161) of huamn IKAP. This sequence is 92% conserved in rat, and 85% conserved in mouse.
The transcription factor NF-kappaB coordinates the activation of numerous genes in response to pathogens and pro-inflammatory cytokines, and is, therefore, vital in the development of acute and chronic inflammatory diseases. NF-kappaB is activated by cytokine-activated IKB kinases (IKKs); IKK-alpha and IKK-beta isozymes are found in large complexes These large, interleukin-1-inducible IKK complexes have been found to contain a new protein, termed IKK-complex-associated protein (IKAP), which can bind NIK and IKKs and assemble them into an active kinase complex. IKAP is a scaffold protein and a regulator for three different kinases involved in pro-inflammatory cytokine signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: elongator acetyltransferase complex subunit 1; Elongator complex protein 1; ELP1; IkappaB kinase complex-associated protein; IKK complex-associated protein; p150
Gene Aliases: DYS; ELP1; FD; IKAP; IKBKAP; IKI3; TOT1
UniProt ID: (Human) O95163
Entrez Gene ID: (Human) 8518
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.