|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide near the N-terminus of human IKAROS.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is HeLa cell lysate.
DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1 Ikaros; CLL-associated antigen KW-6; DNA-binding protein Ikaros; hIk-1; Hs.54452; IKAROS; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Ikaros zinc finger protein; LYF1; Lymphoid transcription factor LyF-1; PRO0758; protein phosphatase 1, regulatory subunit 92; subfamily 1A; zinc finger protein; zinc finger protein, subfamily 1A, 1 (Ikaros); ZNFN1A1
Gene Aliases: CVID13; Hs.54452; IK1; IKAROS; IKZF1; LyF-1; LYF1; PPP1R92; PRO0758; ZNFN1A1
UniProt ID: (Human) Q13422
Entrez Gene ID: (Human) 10320
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