|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the caspase-3 mediated cleavage site.|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-91869 detects IKB alpha cleaved from human samples.
MA1-91869 has been successfully used in Western blot applications.
The MA1-91869 immunogen is: Synthetic peptide corresponding to the caspase-3 mediated cleavage site.
NFKB1 . or NFKB2 . is bound to REL ., RELA ., or RELB . to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Gene Aliases: IKBA; MAD-3; MAD3; NFKBI; NFKBIA
UniProt ID: (Human) P25963
Entrez Gene ID: (Human) 4792
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