|ELISA (ELISA)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human IL-1 alpha|
|Storage buffer||PBS with 4% BSA|
M420AB targets IL-1 alpha in ELISA applications and shows reactivity with Human samples.
The M420AB immunogen is recombinant human IL-1 alpha.
M420AB detects IL-1 alpha which has a predicted molecular weight of approximately 18 kDa.
The M420AB IL1a antibody (biotinylated conjugate of clone B133.5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M700A (clone 2G1). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M420AB (clone ILA8-H12) and antibody M421A (clone ILA9-H18) have successfully been used in combination with recombinant IL1 alpha protein SIL1A in ELISA applications.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Hematopoietin-1; IL-1 alpha; interleukin 1, alpha; Interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
Gene Aliases: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552
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