|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|ELISA (ELISA)||See 12 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-2|
|Purification||Caprylic acid precipitation|
|Storage buffer||PBS with 4% BSA|
MM102B targets IL-2 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM102B immunogen is recombinant mouse IL-2.
MM102B detects IL-2 which has a predicted molecular weight of approximately 17 kDa.
The MM102B mouse IL2 antibody (biotinylated conjugate of clone 1A12) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM101 (clone 5H4). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody MM102B (clone 1A12) and antibody MM101 (clone 5H4) have successfully been used in combination with recombinant mouse IL2 protein SMIL2 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IL-2; Interleukin-2; T-cell growth factor; TCGF
Gene Aliases: Il-2; Il2
UniProt ID: (Mouse) P04351
Entrez Gene ID: (Mouse) 16183
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