|Flow Cytometry (Flow)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Dog , Pig , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||PHA activated T cells.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD25; IL-2 receptor subunit alpha; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA antibody; Il2ra antibody; Interleukin 2 receptor alpha chain antibody; interleukin 2 receptor, alpha; Interleukin-2 receptor subunit alpha; p55; TAC antigen; TAC antigen antibody
Gene Aliases: CD25; IDDM10; IL2R; IL2RA; IMD41; p55; TCGFR
UniProt ID: (Human) P01589
Entrez Gene ID: (Human) 3559
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.