|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||1 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant IL-33 protein|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
A suggested positive control is IL-33 recombinant protein.
Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1-a-b, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappa-B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DVS27-related protein; IL-1F11; IL-33; Interleukin-1 family member 11; Interleukin-33; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules
Gene Aliases: 9230117N10Rik; C9orf26; DVS27; Il-33; IL1F11; IL33; NF-HEV; NFEHEV; NFHEV
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