|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|ELISA (ELISA)||See 4 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-6|
|Purification||Ammonium sulfate precipitation|
MM600D targets IL-6 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM600D immunogen is recombinant mouse IL-6.
MM600D detects IL-6 which has a predicted molecular weight of approximately 22 kDa.
The MM600D mouse IL6 antibody (clone 32C11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM600CB (biotinylated conjugate of clone 20F3). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM600D (clone 32C11) and biotinylated antibody MM600CB (clone 20F3) have successfully been used in combination with recombinant mouse IL6 protein SMIL6 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell hybridoma growth factor; IL-6; Interleukin HP-1; Interleukin-6
Gene Aliases: Il-6; Il6
UniProt ID: (Mouse) P08505
Entrez Gene ID: (Mouse) 16193
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