|ELISA (ELISA)||5 ug/ml|
|Flow Cytometry (Flow)||1/10|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Bovine, Dog, Ferret, Mustelid, Mink, Sheep, Pig, Rabbit|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant ovine IL-8|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This antibody has also been reported to neutralize the bioactivity of ovine IL-8, however prior removal of sodium azide may be necessary for functional assays. Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; IL-8; interleukin 8; Interleukin-8; NAP-1; neutrophil activating peptide 1; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Permeability factor 1; PF1; RPF1
Gene Aliases: AMCF-I; BOS_6776; CXCL8; IL-8; IL8
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