|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|ELISA (ELISA)||See 8 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant human IL-8|
|Storage buffer||PBS with 4% BSA|
M802B targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.
The M802B immunogen is recombinant human IL-8.
M802B detects IL-8 which has a predicted molecular weight of approximately 9 kDa.
The M802B IL8 antibody (biotinylated conjugate of clone I8-S2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M801 (clone 3IL8-H10). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M802B (clone I8-S2) and antibody M801 (clone 3IL8-H10) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; interleukin 8; Interleukin-8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; lymphocyte-derived neutrophil-activating factor; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1
Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1
UniProt ID: (Human) P10145
Entrez Gene ID: (Human) 3576
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