|ELISA (ELISA)||Assay Dependent|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Glycosylated, CHO-derived recombinant human GM-CSF|
|Storage buffer||PBS with 4% BSA|
M501B targets Human GM-CSF in ELISA applications and shows reactivity with Human samples.
The M501B immunogen is glycosylated, CHO-derived recombinant human GM-CSF.
M501B detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The M501B GMCSF antibody (biotinylated conjugate of clone 1089) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M500A (clone 3092). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M501B (clone 1089) and antibody M500A (clone 3092) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications.
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; Molgramostin; Sargramostim
Gene Aliases: CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437
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