|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|ELISA (ELISA)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Glycosylated, CHO-derived recombinant human GM-CSF|
M500A targets Human GM-CSF in ELISA, and WB applications and shows reactivity with Human samples.
The M500A immunogen is glycosylated, CHO-derived recombinant human GM-CSF.
M500A detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The M500A GMCSF antibody (clone 3092) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M501B (biotinylated conjugate of clone 1089). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody M500A (clone 3092) and biotinylated antibody M501B (clone 1089) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications.
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; Molgramostin; Sargramostim
Gene Aliases: CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437
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