|ChIP assay (ChIP)||1 ul|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 579–591 of human IRAK1|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Interleukin-1 receptor-associated kinase 1, in humans, is encoded by the IRAK1 gene which is a serine/threonine-specific kinase. IRAK1 upon stimulation is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. On phosphorylation, IRAK1 recruits the adapter protein PELI1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IL-1 Receceptor Associated Kinase; Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle; Pelle homolog; Pelle-like protein kinase
Gene Aliases: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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