|Western Blot (WB)||0.5-2 µg/ml|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to 16 amino acids near the carboxy terminus of mouse IRAK2a|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
This antibody reacts with all four isoforms or IRAK2a.
A suggested positive control is A-20 cell lysate.
PA5-20164 can be used with blocking peptide PEP-0281.
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappa-B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappa-B activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappa-B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6330415L08Rik, AI649099, IRAK-2; interleukin-1 receptor-associated kinase 2c; Interleukin-1 receptor-associated kinase-like 2; IRAK-2; mu-IRAK-2
Gene Aliases: 6330415L08Rik; AI649099; IRAK-2; Irak2
UniProt ID: (Mouse) Q8CFA1
Entrez Gene ID: (Mouse) 108960
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