|Western Blot (WB)||1:1000-1:2000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide within the human IRE1 alpha protein (within residues 700-800).|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
This antibody does not react with non-human primate samples.
Suggested positive control: Pancreatic islets and insulinoma cells.
IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE1; Ire1-alpha; IRE1a; IRE1P; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1
Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P; RGD1559716
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.