|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
|Immunohistochemistry (IHC)||See 4 publications below|
|Tested Species reactivity||Mouse, Human|
|Published species reactivity||Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal region of the mouse CD11 (b/c) protein within residues 500-600.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Prior to immunostaining paraffin tissues, antigen retrieval with citrate buffer (pH 6.0) is recommended.
Suggested positive control: Raw 264.7 cell lysate.
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11 antigen-like family member C; CD11B; CD11B (p170); CD11bc; CD11C (p150) alpha polypeptide; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor 4; complement receptor type 3; CR-3 alpha chain; CR3A; integrin alpha-M; integrin alpha-X; integrin aX; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); Itgam; Itgax; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor MO1; leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; MAC-1; Mac-1 alpha; MAC1A; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; myeloid membrane antigen, alpha subunit; neutrophil adherence receptor alpha-M subunit; p150 95 integrin alpha chain; SLEB6
Gene Aliases: AI449405; CD11B; CD11b/CD18; CD11C; CR3; CR3A; Cr4; F730045J24Rik; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; N418; SLEB6
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