|Flow Cytometry (Flow)||1 ug/test|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:5,000|
|Immunocytochemistry (ICC)||See 5 publications below|
|Western Blot (WB)||See 4 publications below|
|Blocking Assay (BLOCK)||See 3 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Bovine, Dog, Human, Mouse, Pig, Rat|
|Published species reactivity||Rat , Human Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified NTF97 from bovine erythrocytes.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-070 detects Importin beta 1 from human, canine, bovine, rat and mouse tissues as well as porcine cell lysates. Importin beta 1 is also known as NTF97, Nuclear Transport Factor p97, and karyopherin beta 1. This antibody does not detect bacterially expressed recombinant NTF97.
MA3-070 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~97 kDa protein representing NTF97 in MDBK cell homogenate. Immunofluorescence staining of NTF97 in MDBK cells with MA3-070 results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE. This antibody can inhibit import of nuclear localization sequence-containing proteins in permeabilized cells.
The MA3-070 antigen is purified NTF97 from bovine erythrocytes.
The accumulation of proteins in the nucleus is mediated by short sequences of basic amino acids called nuclear localization sequences (NLSs). These sequences are necessary and sufficient to direct a protein or inert carrier to the nuclear interior. Nuclear protein import is accomplished by two sequential, energy dependent events. The first step, docking at the nuclear pore complex, requires a 54/56 kDa nuclear localization signal receptor (a-karyopherin, importin-a, SRP-a) and the nuclear transport factor p97 (NTF97, b-karyopherin, importin-b). The second step, translocation across the nuclear envelope (NE), requires the GTP-binding protein, Ran/TC4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: importin 1; importin 90; Importin Beta; importin beta-1 subunit; Importin subunit beta-1; Importin-90; Karyopherin; karyopherin (importin) beta 1; karyopherin beta 1; Karyopherin subunit beta-1; karyopherin,beta 1; NTF97; Nuclear factor p97; Nuclear Transport Factor p97; Pore targeting complex 97 kDa subunit; PTAC97; SCG
Gene Aliases: AA409963; IMB1; Impnb; IPO1; IPOB; KPNB1; NTF97
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