|Flow Cytometry (Flow)||1:50-1:100|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This product is a low-endotoxin, preservative-free formulation.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD49d, also known as integrin alpha-4 or VLA-4 subunit alpha, is a ~150kDa single pass type 1 transmembrane glycoprotein that contains seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue through its interaction with MadCam-1, preferentially expressed on Peyerâ€™s patch high endothelial venules and postcapillary venules in lamina propria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 269C wild type; alpha 4 subunit of VLA-4 receptor; alpha-4 subunit of VLA-4 receptor; antigen CD49D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; ITGA4; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
Gene Aliases: CD49D; IA4; ITGA4; Itga4B
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.