|Flow Cytometry (FACS)||1/10 - 1/20|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Tested Species reactivity||Dog|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD11d cell surface antigen, also known as integrin alpha d subunit, is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp. Over expression of CD11d is noted in histiocytic disorders including Hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages with macrophages demonstrating marked erythrocytic phagocytosis. Previously, canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with canine hepatosplenic T-cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp.
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