|Flow Cytometry (Flow)||10 µl for 10^6 cells in 100 µl|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (Agglutination) (WB-Ag)||Assay-dependent|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Osteoclasts from osteoclastomas|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-33578 detects CD51/61 from human samples. MA1-33578 does not react with porcine, lapine or bovine samples.
MA1-33578 has been successfully used in immunoprecipitation and flow cytometry applications. For flow cytometry use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
The MA1-33578 immunogen is Osteoclasts from osteoclastomas.
MA1-33578 recognized the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: antigen CD51; antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GP3A; GPIIIa; integrin alpha V; Integrin alpha-V; integrin alphaVbeta3; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGAV; ITGB3; MSK8; Platelet membrane glycoprotein IIIa; Vitronectin receptor subunit alpha; VNRA; VTNR
Gene Aliases: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; ITGAV; ITGB3; MSK8; VNRA; VTNR
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