|Flow Cytometry (Flow)||1:50-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000-1:5000|
|Immunoprecipitation (IP)||Assay Dependent|
|Tested Species reactivity||Dog, Guinea pig, Human|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human tonsil. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
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Protein Aliases: B-2 integrin; CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; cluster designation 18; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; Integrin beta-2 subunit; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; Lfa1; MF17
Gene Aliases: CD18; ITGB2; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7
UniProt ID: (Human) P05107
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