|Neutralization (Neu)||Assay Dependent|
|Blocking Assay (BLOCK)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human interferon alpha/beta receptor chain 2|
|Purification||Ion-exchange chromatography, Size-exclusion chromatography|
|Storage buffer||PBS with 0.1% BSA|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody neutralizes human IFN-alpha receptor, interacting with the extracellular domain. It binds to human IFN-alpha receptor with high affinity and blocks biological action of Type I IFNs. To measure bioactivity, interferon was titrated with the use of a cytopathic effect inhibition assay, where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha to a 50% endpoint. In this antiviral assay, approximately one unit/ml of interferon was necessary to produce a cytopathic effect of 50%.
Purity is >95% by SDS-PAGE and endotoxin level <1 EU/ug.
Store at -20°C for up to 6 months. For long-term storage, store at -70°C for retention of full activity. Thaw product by incubation in cold water and aliquot into separate vials, adding 0.1% BSA for additional stability. Refreeze product using a dry ice or a dry ice/alcohol bath.
The Type I interferon receptor is a heterodimer consisting of IFNAR1 and IFNAR2. The IFNAR2 subunit provides most of the binding affinity for IFN and IFNAR1 is required for optimum signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: human interferon alpha/beta receptor; IFN-alpha binding protein; IFN-alpha/beta receptor 2; IFN-R-2; IFNABR; IFNAR2; IFNARB; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; Interferon alpha-beta receptor 2; Interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2
Gene Aliases: IFN-alpha-REC; IFN-R; IFNABR; IFNAR2; IFNARB; IMD45
UniProt ID: (Human) P48551
Entrez Gene ID: (Human) 3455
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