|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Western Blot (WB)||2-5 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 290–299 of human junctional adhesion molecule-A|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
JAM-A is a tight junction-associated protein that is a member of the immunoglobulin (Ig) superfamily. JAM-A is expressed on endothelial and epithelial cells, and its sub-cellular distribution is modulated by inflammatory cytokines. It is also expressed on circulating leukocytes and platelets, and lymphoid tissues. It is expressed on various tissues and cells at tight junctions, where it co-localizes with the tight junction proteins Occludin, ZO-1, and Cingulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD321; F11R; JAM; JAM-1; JAM-A; JAM1; JAMA; JCAM; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor
Gene Aliases: CD321; F11R; JAM; JAM1; JAMA; JCAM; KAT; PAM-1; UNQ264/PRO301
UniProt ID: (Human) Q9Y624
Entrez Gene ID: (Human) 50848
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