|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:5000|
|Tested Species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region of the human JMJD1A protein (within residues 250-400).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
The target sequence has 91% sequence homology with rat.
JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: JHDM2A; JHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; jumonji C domain-containing histone demethylase 2A; jumonji domain containing 1; jumonji domain containing 1A; Jumonji domain-containing protein 1A; lysine (K)-specific demethylase 3A; Lysine-specific demethylase 3A; testis specific gene A; testis-specific protein A; TSGA
Gene Aliases: 1700105C21Rik; C230043E16Rik; JHDM2A; JHMD2A; JMJD1; JMJD1A; KDM2A; KDM3A; KIAA0742; TGSA; TSGA
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