|ChIP assay (ChIP)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:10000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region of the human JMJD2C protein (within residues 450-600).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
JMJD2C is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bA146B14.1; GASC-1 protein; GASC1; gene amplified in squamous cell carcinoma 1; Gene amplified in squamous cell carcinoma 1 protein; JHDM3C; JmjC domain-containing histone demethylation protein 3C; JMJD2C; jumonji domain containing 2C; Jumonji domain-containing protein 2C; lysine (K)-specific demethylase 4C; Lysine-specific demethylase 4C; RP11-169L18.1; tudor domain containing 14C
Gene Aliases: 2410141F18Rik; AA517467; GASC1; JHDM3C; JMJD2C; KDM4C; KIAA0780; TDRD14C
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