|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells in 100 µL|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C|
This antibody reacts with rat NKR-PIA, which is expressed by NK cells and NKT cells. Every lot is tested by flow cytometry using freshly harvested rat splenocytes.
Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.
Natural Killer (NK) cells are large, granular lymphocytes found primarily in peripheral blood, where they make up about 10% of the lymphocyte population. Their function is to defend against certain types of tumor cells and virally infected cells. When triggered, the cells release cytotoxic granules which induce either apoptosis or necrosis in the target cell. There are known to be two sets of cell surface receptor on NK cells which recognize target cells with opposing functions. One set, of which an example is NKR-P1 receptors, trigger cytokine release and cell cytotoxicity and the other set, of which Ly49A is an example, inhibit cytokine release. These receptors bind with MHC class I molecules on the surface of healthy cells which inhibits the release of cytokines. In virally infected or cancerous cells, class I MHC molecules are not expressed, inducing the release of cytokine. An activation signal is known to exist, however the mechanics of this signal are not very well understood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen 3.2.3; CD161 antigen-like family member A; CD161a; CLEC5B; hNKR-P1A; Killer cell lectin-like receptor subfamily B member 1A; MGC138614; Natural killer cell surface protein P1-3.2.3; NKR-P1; NKR-P1 3.2.3; NKR-P1A; NKRP1A
Gene Aliases: Klrb1a; NKR-P1A; Nkrp1a
UniProt ID: (Rat) P27471
Entrez Gene ID: (Rat) 362443
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