|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human keratin 20|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon adenocarcinoma.
Keratin 20 / cytokeratin20 (CK20) is a Type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in CK20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CK-20; CK20; cytokeratin 20; Cytokeratin-20; K20; keratin; keratin 20, type I; Keratin, type I cytoskeletal 20; Keratin-20; KRT21; Protein IT; type I cytoskeletal 20
Gene Aliases: CD20; CK-20; CK20; K20; KRT20; KRT21
UniProt ID: (Human) P35900
Entrez Gene ID: (Human) 54474
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