|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human cytokeratin 5|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, breast, prostate or mesothelioma.
Twnety human keratins are divided in to acidic (pl<5.7) and basic (pl > 6.0) subfamilies. Members of the acidic and basic subfamlilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown usefulness of keratins as markers in cancer research and tumor identification. Point mutations in cytokeratin5 gene can cause various types of epidermolysis bullosa simplex. It is expressed in most epithelial and biphasic mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 58 kDa cytokeratin; CK-5; CK5; Cytokeratin-5; DDD; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; Keratin, type II cytoskeletal 5; Keratin-5; KRT5A; Type-II keratin Kb5
Gene Aliases: CK5; DDD; DDD1; EBS2; K5; KRT5; KRT5A
UniProt ID: (Human) P13647
Entrez Gene ID: (Human) 3852
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.