|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunohistochemistry (IHC)||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human keratin 8.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in association with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia. It is present in hepatocellular carcinomas and the majority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cell carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CARD2; CK-8; CK8; CYK8; cytokeratin 8; Cytokeratin-8; K2C8; K8; keratin; keratin 8, type II; Keratin, type II cytoskeletal 8; Keratin-8; KO; type II cytoskeletal 8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8
UniProt ID: (Human) P05787
Entrez Gene ID: (Human) 3856
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